Biochar fungal pellet based biological immobilization reactor efficiently removed nitrate and cadmium

To efficiently and simultaneously remove nitrate (NO₃^--N) and Cd(II) from aqueous solution, a novel type of biochar fungal pellet (BFP) immobilized denitrification bacteria (Cupriavidus sp. H29) composite was used in a bioreactor. The removal performance of the bioreactor R1 for the initial concentration of 27.7 mg L^-1 nitrate and 10.0 mg L^-1 Cd(II) reached 98.1 and 93.9% respectively, and the inoculation of strain H29 in bioreactor R1 significantly enhanced the removal efficiency of contaminants. The 3D-EEM spectra analysis showed that the activity of microorganisms in the bioreactor was higher at a lower concentration of Cd(II). FTIR indicated the effect of functional groups in BFP in bioadsorption of Cd(II). In addition, high-throughput analysis of species composition of the microbial community in the bioreactors at different levels demonstrated that strain H29 played a significant part in the bioreactor. This research provided a perspective for simultaneous restoration of nitrate and heavy metals in wastewater, and also enriched the application of fungal pellet (FP) in reactors.