Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

222
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
222
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

8.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
8.2K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

16.5K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
16.5K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

14.4K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
14.4K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

3.8K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Counterfactual modeling isolates sand mining impacts, revealing it as a key driver of Mekong Delta destabilization.

Science advances·2026
Same author

Genome-Wide Association Study to Identify Novel Genetic Variants Associated With Primary Budd-Chiari Syndrome.

Journal of clinical and experimental hepatology·2026
Same author

Synergistic Zn and Graphene Oxide Enable High-Entropy MOF-74 Nanorods for Multi-Anodic Electrocatalysis.

ACS applied materials & interfaces·2026
Same author

Artificial Intelligence in single-cell and spatial transcriptomics data analyses.

Progress in molecular biology and translational science·2026
Same author

Therapeutic plasma exchange improves short-term survival in patients with acute-on-chronic liver failure: A randomized controlled trial.

Hepatology (Baltimore, Md.)·2026
Same author

Rapid and sensitive detection of cancer-derived small extracellular vesicles using Janus particles.

Nature biomedical engineering·2026

Related Experiment Video

Updated: Oct 3, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

1.6K

pSATdb: a database of mitochondrial common, polymorphic, and unique microsatellites.

Sonu Kumar1, Ashutosh Singh2, Asheesh Shanker3

  • 1Department of Bioinformatics, Central University of South Bihar, Gaya, India.

Life Science Alliance
|February 19, 2022
PubMed
Summary

We developed pSATdb, a novel database detailing mitochondrial microsatellite length variations across species. This resource aids in understanding genetic diversity and species identification using simple sequence repeats.

More Related Videos

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

13.5K
Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

3.9K

Related Experiment Videos

Last Updated: Oct 3, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

1.6K
Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

13.5K
Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

3.9K

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Microsatellites, or simple sequence repeats, are repetitive DNA sequences crucial for phylogeny and species identification.
  • While mitochondrial resources exist, comprehensive data on mitochondrial microsatellite length variation across genera is lacking.
  • Microsatellites exhibit high polymorphism, with length variations useful for genetic studies.

Purpose of the Study:

  • To develop a comprehensive database, pSATdb, for exploring mitochondrial microsatellite length variation between species within a genus.
  • To provide genus-wise information on mitochondrial microsatellite length variation, a unique feature.
  • To facilitate genomics studies by cataloging common, polymorphic, and unique microsatellites.

Main Methods:

  • Identification of 28,710 perfect microsatellites from 5,976 mitochondrial genome sequences.
  • Data compilation from 1,576 genera, encompassing Metazoa and Viridiplantae.
  • Database development (pSATdb) with genus-specific information on microsatellite length variation.

Main Results:

  • pSATdb is the first database offering genus-wise analysis of mitochondrial microsatellite length variation.
  • The database includes data from 1,576 genera, covering both Metazoa and Viridiplantae.
  • Cataloged microsatellites include common, polymorphic, and unique types.

Conclusions:

  • pSATdb provides essential data for comparative genomics and population studies.
  • The database will support research in genetic diversity, mapping, and marker-assisted selection.
  • This resource addresses the need for comprehensive mitochondrial microsatellite information.