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VSNL1 Promotes Cell Proliferation, Migration, and Invasion in Colorectal Cancer by Binding with COL10A1

Chuan He1, Weiguo Liu1, Yan Xiong1

  • 1Department of Digestive Oncology, Medical College of Nanchang University, Cancer Hospital of Jiangxi Province, Nanchang, Jiangxi, China.

Annals of Clinical and Laboratory Science
|February 19, 2022
PubMed
Abstract

Insights

Visinin-like protein 1 (VSNL1) promotes colorectal cancer growth by targeting Collagen Type X Alpha 1 (COL10A1). Targeting this VSNL1/COL10A1 axis may offer new colorectal cancer treatment strategies.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • Colorectal cancer (CRC) remains a significant global health challenge.
  • Understanding the molecular mechanisms driving CRC progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the functional role of the VSNL1/COL10A1 axis in colorectal cancer.
  • To explore VSNL1 and COL10A1 as potential therapeutic targets for CRC.

Main Methods:

  • Bioinformatic analysis of GEO and GEPIA databases for differential gene expression.
  • In vitro assays including cell viability (CCK8), proliferation (EdU), migration, and invasion (Transwell).
  • Molecular techniques such as co-immunoprecipitation (co-IP), RT-PCR, immunohistochemistry, and Western blot.

Main Results:

  • VSNL1 was significantly overexpressed in colorectal cancer tissues.
  • Downregulation of VSNL1 inhibited cancer cell proliferation, migration, and invasion.
  • VSNL1 physically interacted with COL10A1, and COL10A1 upregulation promoted CRC cell growth, reversing VSNL1 knockdown effects.

Conclusions:

  • The VSNL1/COL10A1 axis promotes colorectal cancer progression.
  • VSNL1 acts through COL10A1 to enhance proliferation, migration, and invasion.
  • This axis represents a promising therapeutic target for colorectal cancer treatment.
Keywords:
COL10A1VSNL1colorectal cancer

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