_L-Cysteine functionalized graphene oxide nanoarchitectonics: A metal-free Hg²⁺ nanosensor with peroxidase-like...

Developing non-noble metal, even metal-free chemical sensors for the detection of toxic heavy metal ions is significantly desirable for economically and environmentally sustainable application but has heretofore remained elusive. Herein, a _L-cysteine functionalized graphene oxide nanosheet (CGO) nanoarchitectonics, greenly synthesized by a very simple method at room temperature, was utilized to realize the simultaneous enrichment and colorimetric detection of trace mercury ions (Hg²⁺). It was discovered that CGO, as a nanozyme mimic exhibited greatly enhanced peroxidase-like catalytic activity than the pristine graphene oxide. By exploring the interactions of CGO nanozyme with colorimetric substrate, 3,3',5,5'-tetramethylbenzidine (TMB) and target Hg²⁺ ions, we found that the sensing principle was based mainly on the competitive adsorption between Hg²⁺ ions and TMB over CGO. The pre-capture of Hg²⁺ ions hindered the TMB binding on CGO, resulting in the promoted oxidation of TMB by H₂O₂ to produce more colored oxidation products, from which the colorimetric sensing of Hg²⁺ was realized with a good detection effect on 5 μg L^-1 solution. As an enrichment-sensing integration platform, this metal-free sensor is cost-effective and sensitive, and presents considerable anti-interference ability over other metal ions. Overall, this work not only expands the application of graphene-based materials in colorimetric detection but also provides a general sensing principle to construct highly sensitive sensors.