Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection of H5 subtype avian influenza virus in avian oropharyngeal swab samples using a microfluidic non-competitive fluorescence polarization immunoassay.

The Analyst·2026
Same author

Targeted Intracellular Delivery of Amino Acids to Trophoblast Cells Reveals Proteomic Signatures of Cellular Utilisation.

Biomolecules·2026
Same author

Determination of okadaic acid in scallop using microfluidic-based fluorescence polarization immunoassay.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Structural Elucidation of Azo and Quinoneimine Products Formed in Diazonium-Based Color Reactions of Cannabinoids.

Molecules (Basel, Switzerland)·2026
Same author

CRISPR-Cas3-based editing for targeted deletions in a mouse model of transthyretin amyloidosis.

Nature biotechnology·2026
Same author

Programmable Antigen-Specific Immunity via Self-Adjuvanting Nanovaccines Co-Delivering Immune Modulators.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: Oct 3, 2025

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
06:02

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Published on: March 22, 2022

9.5K

Microfluidic technologies and devices for lipid nanoparticle-based RNA delivery.

Masatoshi Maeki1, Shuya Uno2, Ayuka Niwa2

  • 1Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan; JST PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|February 20, 2022
PubMed
Summary

Microfluidics technology enables precise and reproducible production of RNA-loaded lipid nanoparticles (LNPs) for advanced RNA therapies and vaccines. This method enhances LNP formulation and continuous manufacturing for diverse therapeutic applications.

Keywords:
Lipid nanoparticlesMicrofluidic deviceRNA deliverymRNA vaccine

More Related Videos

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

23.8K
Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries
08:10

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

4.6K

Related Experiment Videos

Last Updated: Oct 3, 2025

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
06:02

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Published on: March 22, 2022

9.5K
Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

23.8K
Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries
08:10

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

4.6K

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Drug Delivery

Background:

  • Messenger RNA (mRNA) vaccines have become crucial for preventing severe COVID-19.
  • Lipid nanoparticles (LNPs) are key delivery vehicles for RNA-based therapies and vaccines.
  • RNA-delivery technology, including mRNA vaccines, has been researched for approximately 30 years.

Purpose of the Study:

  • To review microfluidic technologies for producing RNA-loaded LNPs.
  • To highlight the advantages of microfluidics in LNP manufacturing.
  • To discuss applications of microfluidic-produced LNPs in RNA-based therapy and genome editing.

Main Methods:

  • Summarizing recent advancements in microfluidic devices for LNP production.
  • Analyzing the benefits of microfluidics for LNP formulation and scale-up.
  • Reviewing literature on RNA-loaded LNP applications.

Main Results:

  • Microfluidics offers precise control over LNP size and high reproducibility.
  • Continuous production processes and high-throughput optimization are achievable with microfluidics.
  • Microfluidic LNP production is suitable for diverse RNA-based therapies and vaccines.

Conclusions:

  • Microfluidic technology is a significant advancement for producing RNA-loaded LNPs.
  • This technology facilitates the development of novel RNA-based therapeutics and genome editing tools.
  • Microfluidics enhances the efficiency and scalability of nanomedicine production.