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BCL2L1 is identified as a target of naringenin in regulating ovarian cancer progression

Jing Xu1, Zhe Guo2, Shuang Yuan2

  • 1Department of Obstetrics and Gynecology, Nanyang Central Hospital, No. 312 Gongnong Road, Nanyang, 473005, China. xjfkybq@163.com.

Molecular and Cellular Biochemistry
|February 20, 2022
PubMed

Insights

Naringenin, a natural flavonoid, combats ovarian cancer (OC) by targeting B-cell lymphoma-2 like 1 (BCL2L1). This compound inhibits OC cell proliferation and promotes apoptosis by inactivating the PI3K/Akt/BCL2L1 pathway.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Naringenin is a natural flavonoid with known anti-carcinogenic properties.
  • Ovarian cancer (OC) remains a significant health concern requiring novel therapeutic strategies.

Purpose of the Study:

  • To investigate the anti-tumor effects of naringenin on ovarian cancer progression.
  • To identify the molecular targets and pathways involved in naringenin's action against OC.

Main Methods:

  • Bioinformatic analyses (PharmMapper, SwissTargetPrediction, MalaCards, GEPIA) were used to predict and identify naringenin's targets in OC.
  • Quantitative real-time PCR (qRT-PCR) and Western blot analysis were employed to assess B-cell lymphoma-2 like 1 (BCL2L1) expression.
  • Cell proliferation, apoptosis, and pathway activation assays (CCK-8, EdU, TUNEL, caspase-3, Western blot) were conducted.

Main Results:

  • B-cell lymphoma-2 like 1 (BCL2L1) was identified as a key target of naringenin in ovarian cancer.
  • BCL2L1 expression was found to be upregulated in OC tissues and cells.
  • Naringenin treatment decreased BCL2L1 expression, inhibited cell proliferation, and induced apoptosis in OC cells by inactivating the PI3K/Akt pathway.

Conclusions:

  • Naringenin demonstrates significant anti-tumor activity against ovarian cancer.
  • The anti-cancer effects of naringenin are mediated through the downregulation of BCL2L1 and the inactivation of the PI3K/Akt pathway.
  • Naringenin represents a potential therapeutic agent for ovarian cancer treatment.
Keywords:
BCL2L1NaringeninOvarian cancerPI3K/Akt pathway

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