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Related Experiment Video

Updated: Oct 3, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Selectable Markers to Marker-Free Selection in Rice.

Aditi Sharma1, Ayush Chouhan1, Tarun Bhatt1

  • 1Department of Biotechnology, University Institute of Engineering and Technology, Sector-25, Panjab University, Chandigarh, 160014, India.

Molecular Biotechnology
|February 20, 2022
PubMed
Summary

Rice engineering aims to improve crop resilience against stresses using selection marker genes (SMG). Advanced methods like CRISPR/Cas9 enable efficient, marker-free rice improvement for global food security.

Keywords:
CRISPREngineeringMarker-free selectionRiceSelection marker geneTransformation

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Genetics

Background:

  • Global rice production is hampered by abiotic/biotic stresses and germplasm sensitivity.
  • Cereal biotechnology, particularly rice engineering, is vital for stress tolerance.
  • Plant transformation and selection are key processes in rice engineering.

Purpose of the Study:

  • To review selection marker genes (SMG) used in rice engineering.
  • To discuss strategies for eliminating undesirable foreign DNA post-transformation.
  • To highlight recent advances in marker-free rice improvement.

Main Methods:

  • Review of antibiotic, herbicide, and metabolic SMGs in rice engineering.
  • Analysis of DNA elimination strategies: co-transformation, site-specific recombination, transposons, and CRISPR.
  • Focus on CRISPR/Cas9 for efficient, marker-free gene editing.

Main Results:

  • SMGs are essential for initial selection but undesirable for commercial rice.
  • Various methods exist to remove foreign DNA after successful transformation.
  • CRISPR/Cas9 offers a simple, efficient approach for marker-free gene modification.

Conclusions:

  • CRISPR/Cas9 technology is pivotal for developing 'clean gene' rice.
  • Direct application in embryos facilitates rapid, marker-free gene editing.
  • Recent advances in rice engineering hold significant potential for crop improvement.