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Updated: Oct 3, 2025

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
Published on: September 13, 2022
The spindle checkpoint proteins BUB1 and BUBR1: (SLiM)ming down to the basics
Sabine Elowe1, Victor M Bolanos-Garcia2
1Département de Pédiatire, Faculté de Médicine, Université Laval; Axe de réproduction, santé de la mère et de l'enfant, Centre de recherche-CHU de Québec and Centre de recherche sur le cancer de l'Université Laval, Québec QC G1R 2J6, Quebec, Canada; PROTEO, Regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines, Québec, Quebec, Canada.
Abstract:
Benzimidazole 1 (BUB1) and budding uninhibited by benzimidazole 1-related 1 (BUBR1) are multidomain paralogs with key roles in chromosome alignment during mitosis and the spindle assembly checkpoint (SAC), an evolutionarily conserved signaling pathway that monitors errors in chromosome segregation during cell division in eukaryotes. Although BUB1 and BUBR1 share a similar domain organization and short linear interaction motifs (SLiMs), they control distinct aspects of chromosome congression and the SAC. Here we discuss the roles of BUB1 and BUBR1 SLiMs in mitosis and complement this with additional insights gleamed from studying their evolution. We show that BUB1 and BUBR1 SLiMs form highly specific interactions that are carefully orchestrated in space and time and contend that they define BUB1 and BUBR1 as organizing hubs that drive SAC signaling and ensure genome stability.
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