Comparative analysis of nitrogen concentrations and sources within a coastal urban bayou watershed: A multi-tracer...

Fate and transport of nitrogen (N) in urban coastal watersheds continues to draw research interest due to serious impacts of N pollution and complexities with N sources and transport pathways. In this study, we used multiple tracers for source identification of N pollution (¹⁵N isotope in nitrate and chemical sewage tracers in water) and waters (using isotopes of ¹⁸O and ²H in water) in a coastal northwest Florida U.S.A. urban bayou fed by two contrasting streams, namely Jackson Creek traversing a dense residential area and Jones Creek flowing mainly through a wetland preserve. Results showed that the slightly higher δ¹⁵N-NO₃ ^- values in Jones Creek and the bayou were insufficient to distinguish N sources; yet the different chemical sewage tracer concentrations (e.g., sucralose, carbamazepine and sulfamethoxazole) clearly demonstrated the major N source from leaking septic tanks in the Jackson Creek sub-basin but not in the Jones Creek sub-basin. The higher concentrations of nitrate, which constituted over 98% of dissolved inorganic N in Jackson Creek, support active nitrification in sandy soils and steep terrain while higher δ¹⁵N-NO₃ ^- and much lower nitrate in Jones Creek are likely associated with denitrification in dense vegetative wetland and riparian zones. Episodic high nitrate concentrations and δ¹⁸O values in Jackson Creek preceded by periods of little rainfall indicated that the creek was sustained by subsurface flow with a steady input of nitrate. This study demonstrated the connection of land use and stormwater runoff generation to the forms of N entering urban waterways, the utility of N sourcing approaches, and the value of watershed-scale assessments for developing strategies to limit N loadings in urban settings.