Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of cat insulin.

G Halldén, G Gafvelin, V Mutt

    Archives of Biochemistry and Biophysics
    |May 15, 1986
    PubMed
    Summary

    Researchers isolated and characterized cat insulin, finding four differences from human insulin. Notably, cat insulin has a unique histidine at position A18, potentially affecting receptor binding.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1.

    Allergy·2016
    Same author

    IgG4 but no IgG1 antibody production after intralymphatic immunotherapy with recombinant MAT-Feld1 in human.

    Allergy·2016
    Same author

    Altered immunoregulatory profile during anti-tumour necrosis factor treatment of patients with inflammatory bowel disease.

    Clinical and experimental immunology·2012
    Same author

    The oncolytic adenovirus AdΔΔ enhances selective cancer cell killing in combination with DNA-damaging drugs in pancreatic cancer models.

    Gene therapy·2011
    Same author

    An oncolytic adenovirus defective in pRb-binding (dl922-947) can efficiently eliminate pancreatic cancer cells and tumors in vivo in combination with 5-FU or gemcitabine.

    Cancer gene therapy·2011
    Same author

    Treatment with a Fel d 1 hypoallergen reduces allergic responses in a mouse model for cat allergy.

    Allergy·2010

    Area of Science:

    • Biochemistry
    • Comparative Endocrinology

    Background:

    • Insulin is a vital hormone regulating blood glucose.
    • Understanding insulin structure variations across species is key to comprehending its function and evolution.

    Purpose of the Study:

    • To determine the primary structure of cat insulin.
    • To compare cat insulin with other known insulin sequences, including human insulin.
    • To investigate potential functional implications of structural differences.

    Main Methods:

    • Isolation of cat insulin.
    • Determination of the primary amino acid sequences for both chains of cat insulin.
    • Comparative sequence analysis with other mammalian insulins.

    Main Results:

    • The primary structure of cat insulin was elucidated.
    • Cat insulin differs from human insulin at four amino acid positions: A8 (Ala), A10 (Val), A18 (His), and B30 (Ala).
    • Position A18 (His) in cat insulin is unique among known insulin structures.

    Conclusions:

    • Cat insulin possesses a distinct primary structure compared to human insulin.
    • The unique histidine at position A18 in cat insulin may influence receptor binding properties due to its proximity to the receptor interaction site.
    • Further studies are warranted to explore the functional consequences of these structural variations.

    Related Experiment Videos