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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Oct 3, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Hemp Genome Editing-Challenges and Opportunities.

Donal Shiels1, Barbara Doyle Prestwich1, Okjae Koo2

  • 1School of Biological Earth and Environmental Sciences, Environmental Research Institute, University College Cork, Cork, Ireland.

Frontiers in Genome Editing
|February 21, 2022
PubMed
Summary

Genome editing offers a path to non-transgenic hemp with improved traits for medicine and industry. This approach leverages AI and genomic data to enhance valuable cannabinoid production and environmental benefits.

Keywords:
HEMPartificial intelligence for crop improvementcannabinoidsgenome editingnext generation technologiesnon-transgenictissue culture

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Area of Science:

  • Agricultural Science
  • Plant Biotechnology
  • Genomics

Background:

  • Hemp (Cannabis sativa L.) is a versatile crop with significant applications in medicine, fiber, food, and biocomposites.
  • Hemp cultivation offers environmental benefits, including carbon sequestration and soil health improvement.
  • The multibillion-dollar market for hemp-derived cannabinoids is expanding rapidly.

Purpose of the Study:

  • To explore the application of advanced genome editing tools for improving industrially desirable traits in non-transgenic hemp.
  • To leverage recent advancements in genome sequencing and Artificial Intelligence (AI) for trait discovery in hemp.
  • To address regulatory challenges associated with genetically modified crops by focusing on non-transgenic genome editing.

Main Methods:

  • Utilizing high-quality Cannabis genome sequences from various strains.
  • Applying advanced genome editing technologies to target specific beneficial traits.
  • Integrating AI-mediated trait discovery platforms for enhanced crop improvement.
  • Examining interdisciplinary approaches from existing plant species technologies.

Main Results:

  • Genome editing presents a viable strategy for developing non-transgenic hemp with enhanced properties.
  • AI and genomic data accelerate the discovery and improvement of valuable hemp traits.
  • Non-transgenic genome editing can overcome regulatory hurdles, facilitating wider adoption.

Conclusions:

  • Advanced genome editing tools hold significant potential for optimizing hemp for medicinal and industrial applications.
  • The development of non-transgenic, genome-edited hemp can contribute to sustainable agriculture and human welfare.
  • Further research integrating interdisciplinary approaches is crucial for realizing the full potential of hemp improvement.