Multicolor strategies for investigating clonal expansion and tissue plasticity
L Dumas1, S Clavreul1, F Michon1
1Institute for Neurosciences of Montpellier (INM), Univ Montpellier, INSERM, Montpellier, France.
Cellular and Molecular Life Sciences : CMLS
|February 21, 2022
Summary
Multicolor lineage tracing in mouse models reveals cellular choreography during organogenesis. These advanced tools, including Brainbow and StarTrack, are crucial for understanding tissue development and disease.
Area of Science:
- Developmental Biology
- Genetics
- Bioimaging
Background:
- Understanding organismal complexity necessitates multicellular lineage tracing.
- Fluorescent reporter protein strategies are key to multicolor lineage tracing.
Purpose of the Study:
- To review multicolor lineage tracing strategies, focusing on mouse models.
- To highlight applications in organogenesis, tissue morphogenesis, and disease.
Main Methods:
- Description of classical (MADM, Brainbow) and acute (StarTrack, CLoNe) transgenesis methods.
- Discussion of multi-reporter genetic strategies in various animal and cellular models.
- Focus on applications in brain, intestine, skin, vascular, hematopoietic, and immune systems.
Main Results:
- Multicolor mouse models enable clonal analyses at the single progenitor scale.
- Fate mapping strategies reveal fine cellular choreography in tissue morphogenesis.
- Applications span understanding brain cytoarchitecture in health and disease.
Conclusions:
- Multicolor lineage tracing provides unprecedented insights into developmental processes.
- Technological advances in imaging and analysis enhance data exploitation for biological discovery.
