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Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

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Development and Forensic Application of EX20+30Y Kit.

Xu-Ming Zhou¹, Deng-Xing Gao², Fa-Yuan Li³

¹Expert Evidence Center of Shangrao Public Security, Shangrao 334000, Jiangxi Province, China.

Fa Yi Xue Za Zhi

|February 21, 2022

Summary

A new multiplex PCR system (EX20+30Y) accurately genotypes 19 autosomal and 30 Y-STR loci plus gender. This highly sensitive forensic tool shows promise for casework and genetic analysis.

Keywords:

Y chromosome autosome forensic genetics kit short tandem repeat

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Area of Science:

Forensic Genetics
Molecular Biology
DNA Analysis

Background:

Multiplex PCR systems are crucial for efficient DNA profiling in forensics.
Existing systems may have limitations in the number of loci or sensitivity.
Developing advanced multiplex assays enhances forensic investigation capabilities.

Purpose of the Study:

To develop and validate a novel multiplex PCR amplification system, named EX20+30Y.
To combine the analysis of 19 autosomal Short Tandem Repeat (STR) loci, 30 Y-STR loci, and a gender indicator in a single assay.
To assess the forensic applicability and performance of the developed system.

Main Methods:

Construction of a six-color fluorescence-labeled multiplex amplification system.
Utilizing 19 autosomal STR loci, 30 Y-STR loci, and a gender indicator.
Evaluation of sensitivity, mixed sample detection, species specificity, balance, direct amplification, sample applicability, and anti-inhibition properties using various sample types.

Main Results:

The EX20+30Y system achieved accurate genotyping for 210 unrelated individuals.
Demonstrated high sensitivity with a detection limit of 0.125 ng for standard samples.
Successfully genotyped 69 daily case samples and showed resistance to inhibitors in standard samples.

Conclusions:

The developed EX20+30Y multiplex amplification system enables accurate combined examination of 19 autosomal and 30 Y-STR loci plus gender.
The system exhibits high sensitivity and robust performance, indicating significant forensic application potential.
This assay offers an advanced tool for forensic DNA profiling and identification.