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Updated: Oct 3, 2025

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
ASES: visualizing evolutionary conservation of alternative splicing in proteins
Diego Javier Zea1, Hugues Richard2, Elodie Laine1
1Laboratoire de Biologie Computationnelle et Quantitative (LCQB), CNRS, IBPS, Sorbonne Université, 75005 Paris, France.
Summary:
ASES is a versatile tool for assessing the impact of alternative splicing (AS), initiation and termination of transcription on protein diversity in evolution. It identifies exon and transcript orthogroups from a set of input genes/species for comparative transcriptomics analyses. It computes an evolutionary splicing graph, where the nodes are exon orthogroups, allowing for a direct evaluation of AS conservation. It also reconstructs a transcripts' phylogenetic forest to date the appearance of specific transcripts and explore the events that have shaped them. ASES web server features a highly interactive interface enabling the synchronous selection of events, exons or transcripts in the different outputs, and the visualization and retrieval of the corresponding amino acid sequences, for subsequent 3D structure prediction.
Availability And Implementation:
http://www.lcqb.upmc.fr/Ases.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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