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Updated: Oct 3, 2025

Author Spotlight: Reliable and Reproducible In Vitro Assessment of Drug Impact on Rat Intestinal Tubes
Published on: July 26, 2024
Exploring the Predictive Power of the In Situ Perfusion Technique towards Drug Absorption: Theory, Practice, and
Bart Hens1, Isabel Gonzalez-Alvarez2, Marival Bermejo2
1Drug Product Design, Pfizer, Sandwich, Kent, CT13 9NJ, United Kingdom.
Abstract:
Considering the broad applications and popularity, the in situ perfusion technique is an established and interesting approach to evaluate the absorption mechanisms of drug molecules in specific regions of the intestinal tract. Compared to perfusion studies in humans, this surrogate model shows several familiar characteristics making it interesting to apply this technique in rats in the non-clinical stage of drug product development. The differences in gastrointestinal (GI) anatomy and physiology between rats and humans are thoroughly discussed in the present review. Moreover, an in-depth overview of the Doluisio (i.e., closed-loop) versus the single-pass intestinal perfusion (i.e., open-loop) technique is shown. Finally, applications and future perspectives of the technique are presented.
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