Rapid Detection of the Recently Identified Turfgrass Pathogen Magnaporthiopsis meyeri-festucae Using Recombinase...

Area of Science:

• Plant Pathology
• Molecular Diagnostics
• Turfgrass Science

Background:

• Magnaporthiopsis meyeri-festucae is an emerging root-infecting pathogen affecting fine fescue turfgrass.
• Accurate and rapid identification is crucial for effective management due to its distinct characteristics compared to related pathogens like M. poae.
• Current diagnostic methods may not be suitable for rapid field application.

Purpose of the Study:

• To develop a rapid detection method for Magnaporthiopsis meyeri-festucae using recombinase polymerase amplification (RPA).
• To provide turfgrass managers with a tool for quick in-field identification.
• To facilitate further epidemiological research on M. meyeri-festucae.

Main Methods:

• Developed and tested a recombinase polymerase amplification (RPA) assay targeting M. meyeri-festucae.
• Evaluated assay specificity using three M. meyeri-festucae isolates and eight isolates from four related Magnaporthiopsis species.
• Visualized RPA results using SYBR-Safe dye for rapid detection on turfgrass roots, including samples subjected to inoculation and grinding.

Main Results:

• The RPA assay effectively detected M. meyeri-festucae on turfgrass roots with no false positives or negatives.
• The assay demonstrated specificity, differentiating M. meyeri-festucae from other Magnaporthiopsis species, though sensitivity was lower than PCR.
• Successful detection after direct inoculation and grinding of roots indicates potential for rapid field diagnostics.

Conclusions:

• A fast and accurate RPA-based detection method for M. meyeri-festucae has been successfully developed.
• This method holds significant potential as a rapid field diagnostic tool for turfgrass managers.
• The assay will support improved management strategies and further research on this emerging turfgrass pathogen.