Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Copper oxide nanoparticles: from EU-regulatory landscape and mechanistic toxicity towards a cuproptosis-linked adverse outcome pathway.

Archives of toxicology·2026
Same author

A combined in silico and in vitro new approach methodology for early detection of liver steatogenic chemicals.

Chemico-biological interactions·2026
Same author

A pipeline for developing AI-driven models to predict molecular initiating events: a case study on neural tube defects.

Journal of cheminformatics·2026
Same author

Variability and uncertainty of data from genotoxicity test guidelines: what we know and why it matters.

Archives of toxicology·2026
Same author

Structure-Activity Relationship of Protein-Mediated Molecular Initiating Events of Organ-Specific Toxicities.

Chemical research in toxicology·2025
Same author

Genetic and epigenetic biomarkers in human biomonitoring: why needed and how can Oxford Nanopore sequencing contribute?

Frontiers in public health·2025

Related Experiment Video

Updated: Oct 3, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.5K

In Silico Methods for Chromosome Damage.

Diego Baderna1, Ilse Van Overmeire2, Giovanna J Lavado1

  • 1Istituto Di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Lombardia, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2022
PubMed
Summary

In silico tools can predict chromosome damage in pharmaceuticals, aiding regulatory toxicology. A battery of these computational methods offers valuable insights, especially when used together.

Keywords:
Chromosome damageGenotoxicityMetaphase chromosome aberration testMicronucleus testQSAR modelsStructural alerts; In silico profilers

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

3.7K
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
07:54

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

17.2K

Related Experiment Videos

Last Updated: Oct 3, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.5K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

3.7K
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
07:54

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

17.2K

Area of Science:

  • Toxicology
  • Computational chemistry
  • Pharmacology

Background:

  • Genotoxicity is critical for human health assessment in pharmaceutical regulation.
  • Assessing a compound's potential for gene mutations and chromosome damage is essential.
  • Traditional in vitro and in vivo methods for chromosome damage assessment exist.

Purpose of the Study:

  • To evaluate freely available in silico tools for predicting chromosome damage.
  • To apply a battery of these computational tools to a pharmaceutical dataset.
  • To analyze the utility of in silico approaches for genotoxicity testing.

Main Methods:

  • A collection of freely accessible in silico chromosome damage prediction tools was utilized.
  • These tools were applied to a dataset comprising various pharmaceuticals.
  • The outcomes from the in silico battery were analyzed and discussed, including a case study on coumarin.

Main Results:

  • In silico tools for chromosome damage prediction were applied to pharmaceutical compounds.
  • Examples of diverse outcomes from the computational battery were presented.
  • A detailed case study using coumarin illustrated the application and results of the in silico approach.

Conclusions:

  • In silico tools for chromosome damage, while less developed than mutagenicity predictors, offer significant value.
  • Combining multiple in silico tools in a battery enhances their predictive power.
  • These computational methods provide valuable toxicological data for pharmaceuticals, especially in early assessments.