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Cas9-Mediated Targeted Mutagenesis in Plants.

Quentin M Dudley1,2, Oleg Raitskin1, Nicola J Patron3

  • 1Earlham Institute, Norwich Research Park, Norwich, Norfolk, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2022
PubMed
Summary

This study presents a rapid method for designing and testing Cas9 gene editing constructs in plants. The technique enables efficient targeted mutations for crop development and basic plant science research.

Keywords:
Arabidopsis thalianaCRISPRCas9Genome editingGenome engineeringMutagenesisNicotiana benthamianaProtoplastsgRNA

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