Plant X-tender Toolbox for the Assembly and Expression of Multiple Transcriptional Units in Plants

Plant synthetic biology requires the design of plant expression vectors with multiple transcriptional units, which can be challenging. Here we describe the use of Plant X-tender toolbox complemented with a plant grammar implemented in GenoCAD for design, cloning and delivery of several transcriptional units into the plant genome. Plant X-tender toolbox consists of a set of plant expression vectors and the protocols for the most efficient cloning of multiple transcriptional units into this novel vector set. Together with the plant grammar implemented in GenoCAD, the presented strategy allows the users to quickly design genetic modules and assemble them into Plant X-tender expression vectors for in planta functional studies or synthetic biology applications.