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MicroRNA-183 accelerates the proliferation of hepatocyte during liver regeneration through targeting programmed cell

Fangxing Hou1, Xing Li2, Yanfeng Wang3

  • 1Queen Mary School, Nanchang University, Nanchang, Jiangxi, China.

Genes & Genomics
|February 22, 2022
PubMed
Abstract

Insights

MicroRNA-183 (miR-183) promotes liver regeneration by targeting programmed cell death protein 6 (PDCD6). Upregulating miR-183 enhances liver regeneration, while inhibiting it has the opposite effect.

Area of Science:

  • Hepatology and molecular biology
  • Cellular mechanisms of regeneration

Background:

  • Liver regeneration is a complex process influenced by microRNAs (miRs).
  • miR-183 is upregulated during liver regeneration following partial hepatectomy.

Purpose of the Study:

  • To investigate the mechanism by which miR-183 influences liver regeneration.
  • To explore the relationship between miR-183 and programmed cell death protein 6 (PDCD6) in liver regeneration.

Main Methods:

  • Partial hepatectomy (PH) and cell transfection in rats and hepatocytes.
  • Measurement of miR-183 and PDCD6 levels, histopathology, and liver/body weight ratio.
  • Luciferase activity assays to confirm miR-183 targeting of PDCD6.
  • MTT and flow cytometry assays to assess cell viability, apoptosis, and cell cycle.

Main Results:

  • miR-183 expression inversely correlated with PDCD6 levels during liver regeneration.
  • Enhanced miR-183 increased liver/body weight ratio; inhibition decreased it.
  • miR-183 targeted PDCD6, affecting hepatocyte viability, apoptosis, and cell cycle progression.

Conclusions:

  • miR-183 promotes liver regeneration.
  • The mechanism involves targeting programmed cell death protein 6 (PDCD6).
Keywords:
Hepatic indexHepatocytesLiver regenerationPartial hepatectomymicroRNA-183

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