Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

72.7K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
72.7K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.7K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane protein structure and dynamics probed by MicroED.

Biochemical Society transactions·2026
Same author

Rapid Structural Analysis of Natural Products Using MicroED.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Discovery of Oxyacanthine Dihydrochloride Monohydrate Polymorphs from Obfuscated Samples by Microcrystal Electron Diffraction.

ChemMedChem·2025
Same author

pH-dependent regulation in SLC38A9.

bioRxiv : the preprint server for biology·2025
Same author

Chemical and ribosomal synthesis of atropisomeric and macrocyclic peptides with embedded quinolines.

Nature chemistry·2025
Same author

The 2024 challenges in structural biology summit.

Structural dynamics (Melville, N.Y.)·2025

Related Experiment Video

Updated: Oct 2, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.5K

Studying membrane proteins with MicroED.

Marc J Gallenito1, Tamir Gonen1,2,3

  • 1Department of Biological Chemistry, University of California, Los Angeles, Los Angeles CA 90095, U.S.A.

Biochemical Society Transactions
|February 22, 2022
PubMed
Summary
This summary is machine-generated.

Microcrystal electron diffraction (MicroED) offers atomic resolution for small crystals. This review highlights MicroED

Keywords:
MicroEDcryo-EMmicrocrystal electron diffractiontransmembrane proteins

More Related Videos

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

6.1K

Related Experiment Videos

Last Updated: Oct 2, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.5K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

6.1K

Area of Science:

  • Structural Biology
  • Biochemistry
  • Biophysics

Background:

  • Understanding disease mechanisms requires investigating biological macromolecule structures.
  • Various structural biology techniques exist, with electron cryomicroscopy (cryo-EM) and microcrystal electron diffraction (MicroED) yielding high-resolution biomolecule structures.
  • MicroED, since 2013, has excelled at solving structures from minuscule crystals.

Purpose of the Study:

  • To review the progression of electron crystallography from 2D to 3D crystals using MicroED.
  • To explore the application of MicroED to membrane proteins, a challenging frontier.
  • To discuss specific membrane protein families successfully studied by MicroED.

Main Methods:

  • Electron crystallography
  • Microcrystal electron diffraction (MicroED)
  • Analysis of membrane protein structures

Main Results:

  • MicroED has achieved atomic resolution for biological and small molecules using small crystals.
  • Significant progress has been made in electron crystallography, leading to MicroED.
  • Four distinct membrane protein families have had representatives solved using MicroED.

Conclusions:

  • MicroED is a powerful technique for high-resolution structure determination.
  • Further optimization of sample handling and imaging is needed for MicroED of membrane proteins.
  • MicroED is expanding its reach to complex biological systems like membrane proteins.