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Updated: Oct 2, 2025
Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
Published on: August 1, 2022
Development of a decellularized hypopharynx with vascular pedicle scaffold for use in reconstructing hypopharynx
Nan Hou1, Die Lv1,2, Xiaoli Xu1
1Department of Otorhinolaryngology Head and Neck Surgery, Clinical Medical College and The First Affiliated Hospital, Chengdu Medical College, Chengdu City, China.
Background:
Hypopharynx reconstruction after hypopharyngectomy is still a great challenge. Perfusion decellularization is for extracellular matrix (ECM) scaffolding and had been used in organ reconstruction. Our study aimed to prepare an acellular, natural, three-dimensional biological hypopharynx with vascular pedicle scaffold as the substitute materials to reconstruct hypopharynx.
Result:
Scanning electron microscope and histology staining showed that the decellularized hypopharynx with vascular pedicle scaffold retained intact native anatomical ECM structure. Myoblasts were observed on the recellularized scaffolds with bone marrow mesenchymal stem cells induced by 5-azacytidine implanted in the rabbit greater omentum by immunohistochemical analysis.
Conclusion:
The decellularized hypopharynx with vascular pedicle scaffold prepared by detergent perfusion in our study has a potential to be an alternative material to pharynx reconstruction.
