Related Experiment Video
Updated: Jun 17, 2026
10:11
Cell Co-culture Patterning Using Aqueous Two-phase Systems
Published on: March 26, 2013
18.6K
Downstream processing of virus-like particles with aqueous two-phase systems: Applications and challenges
Hui Yi Leong1, Xiao-Qian Fu1, Pau Loke Show2
1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, P. R. China.
Journal of Separation Science
|February 22, 2022
Summary
Aqueous two-phase systems offer a safe, cost-effective method for purifying virus-like particles (VLPs). This review explores their application in VLP vaccine production, addressing challenges and future potential.
Area of Science:
- Biotechnology
- Vaccine Development
- Bioprocessing
Background:
- Recombinant virus-like particle (VLP)-based vaccines show high safety and efficacy for infectious and chronic diseases.
- Efficient downstream processing is crucial for affordable and safe VLP vaccine production.
- Aqueous two-phase systems (ATPS) are recognized for their efficiency, environmental friendliness, and scalability in biomolecule recovery.
Purpose of the Study:
- To review the current applications of ATPS in the downstream processing of VLPs.
- To highlight the challenges and future outlook of using ATPS for VLP purification.
- To bridge the research gap in VLP downstream production for vaccine development.
Main Methods:
- Literature review of ATPS applications in VLP purification.
- Analysis of ATPS efficiency, cost-effectiveness, and scalability.
- Identification of challenges and future research directions in VLP downstream processing.
Main Results:
- ATPS present a promising, scalable, and cost-effective technology for VLP purification.
- Successful applications of ATPS in recovering and purifying various recombinant VLPs have been demonstrated.
- Key challenges include optimizing phase composition and system integration for industrial scale.
Conclusions:
- ATPS are a viable and advantageous technology for the downstream processing of recombinant VLPs.
- Further research is needed to overcome existing challenges and fully realize the potential of ATPS in VLP vaccine production.
- This review provides a comprehensive overview to guide future research and development in VLP manufacturing.
Keywords:
aqueous two-phase systemscontinuous bio-manufacturingextractionintegrated processesvirus-like particlesMore Related Videos
Related Concept Videos
01:09Colloidal precipitates
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
01:23Vaccine Production
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
01:29Downstream Processing
Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...