Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revealing the pH-Dependent Adsorption Dynamics of Tetracycline Hydrochloride on Phosphoric Acid-Activated Corncob Biochar.

Materials (Basel, Switzerland)·2026
Same author

Plasma-derived exosomes from Graves' orbitopathy: Pathogenic entities causing tissue lesions.

Experimental eye research·2026
Same author

Perineural invasion in head and neck squamous cell carcinoma: Neuro-immune mechanisms driving immunotherapy resistance and emerging therapeutic strategies.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2026
Same author

Transcriptional Regulation of Starch Biosynthesis in Sorghum Grain by a MIKC-Type MADS-Box Transcription Factor: An In Vitro Analysis.

Plants (Basel, Switzerland)·2026
Same author

Lived Experience of Extracorporeal Membrane Oxygenation Survivors: A Phenomenological Study.

Nursing in critical care·2026
Same author

Targeting the plasticity of intestinal neutrophils: bidirectional regulation strategies by natural products.

Frontiers in immunology·2026

Related Experiment Video

Updated: Oct 2, 2025

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

10.4K

A yeast-based biosensor for silver nanoparticle accumulation and cellular dissolution.

Anqi Sun1, Wen-Xiong Wang2

  • 1Division of Life Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong, China; School of Energy and Environment and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, China.

Biosensors & Bioelectronics
|February 22, 2022
PubMed
Summary

A novel biosensing technique using adenine deficient yeast quantifies ultra-small silver nanoparticles (AgNPs) and their dissolution. This method differentiates AgNP sizes and monitors cellular AgNP breakdown, crucial for biological assessments.

Keywords:
Ag NPAg ionAutofluorescenceBiosensorsYeast

More Related Videos

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

11.9K
Formulation and Characterization of Bioactive Agent Containing Nanodisks
07:58

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

1.3K

Related Experiment Videos

Last Updated: Oct 2, 2025

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

10.4K
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

11.9K
Formulation and Characterization of Bioactive Agent Containing Nanodisks
07:58

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

1.3K

Area of Science:

  • Nanotechnology
  • Biosensing
  • Environmental Science

Background:

  • Increasing applications of silver nanoparticles (AgNPs) necessitate accurate quantification and cellular dissolution monitoring for biological safety.
  • Existing methods may lack sensitivity for ultra-small AgNPs or fail to differentiate size-dependent responses.
  • Dissolved silver ions (Ag+) interfere with biological processes, making their quantification critical.

Purpose of the Study:

  • To develop a novel, sensitive technique for quantifying ultra-small AgNPs and their cellular dissolution.
  • To utilize adenine deficient yeast [Ade(-) yeast] as a biosensor for AgNPs.
  • To differentiate between ultra-small and larger AgNPs based on their biological response.

Main Methods:

  • Developed a biosensing method based on Ag+ inhibiting Zn2+-directed fluorescence in Ade(-) yeast.
  • Optimized yeast biomass and Zn2+ concentration for maximal AgNP sensitivity.
  • Applied the method to quantify ultra-small AgNPs and assess their dissolution within yeast cells.

Main Results:

  • The lowest detectable dissolved Ag+ was 3.77 ng Ag/μg P, and the lowest detectable concentration of 7 nm AgNPs was 8.9 μg/L.
  • Ade(-) yeast selectively responded to ultra-small AgNPs (4.5-9 nm), acting as a screener to distinguish them from larger AgNPs (16-26 nm).
  • Over 78% of internalized 7 nm AgNPs dissolved into Ag+ within yeast cells.

Conclusions:

  • A novel Ade(-) yeast-based biosensing system effectively quantifies ultra-small AgNPs and their dissolution.
  • This system can screen ultra-small AgNPs and differentiate them from larger nanoparticles.
  • The findings provide a valuable tool for assessing the biological impact and fate of ultra-small AgNPs.