An eco- friendly, selective, and sensitive spectrofluorimetric method for the quantification of Dolutegravir in its...

This study is concerned with assessing the bulk and tablet dosage forms of Dolutegravir (DTG) by means of a novel yet simple environmentally friendly spectrofluorimetric method that features fast response and high sensitivity compared to the time consuming HPLC methods and the lower sensitivity spectrophotometric ones. The method relies mainly on measuring the native fluorescence of Dolutegravir in water at an emission of 415 nm after excitation at 262 nm. The method shows rectilinear fluorescence-concentration relation over Dolutegravir concentration range of 0.2-1.2 µg/mL at the emission maxima, with detection limit of 0.020 µg/mL and quantification limit of 0.061 µg/mL. The results of the proposed method were compared with those obtained by applying the comparison method and the two sets coincided harmoniously. In addition, the method was validated in accordance with ICH guidelines for linearity, accuracy, precision, specificity, and robustness.