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Updated: Oct 2, 2025

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

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Published on: October 25, 2016

CD161 expression defines new human γδ T cell subsets.

Amali Karunathilaka¹, Samuel Halstrom², Patricia Price²

¹Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.

Immunity & Ageing : I & A

|February 23, 2022

Summary

This summary is machine-generated.

New research identifies novel gamma delta (γδ) T cell subsets based on CD161 expression. These subsets may play a role in chronic inflammatory diseases like bronchiectasis.

Keywords:

Bronchiectasis CD161 Cellular immunity FlowSOM High dimensional flow cytometry Immune checkpoint Unsupervised clustering γδ T cell γδ T cell multifunctionality γδ T cell subsets

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Expansion and Enrichment of Gamma-Delta γδ T Cells from Apheresed Human Product

Expansion and Enrichment of Gamma-Delta γδ T Cells from Apheresed Human Product

Published on: September 22, 2021

Area of Science:

Immunology
Cell Biology

Background:

Gamma delta (γδ) T cells are crucial for immune defense and maintaining homeostasis.
Their heterogeneity, precise functions, and roles in health and disease require further elucidation.

Purpose of the Study:

To identify novel γδ T cell subsets, particularly in middle-to-old age individuals.
To investigate the role of CD161 expression in defining γδ T cell phenotypes and their potential link to disease.

Main Methods:

Multi-parametric flow cytometry was employed to analyze γδ T cell populations.
Dimensionality reduction, unsupervised clustering, and self-organizing maps (SOM) were utilized for subset identification.
Analysis included comparison between healthy elderly individuals and patients with bronchiectasis.

Main Results:

Novel γδ T cell subsets were identified, primarily distinguished by CD161 expression levels.
Vδ1+ γδ T cells predominantly showed a terminally differentiated phenotype, while Vδ1- cells displayed an early memory phenotype.
Elevated Vδ1+ terminally differentiated effector memory cells were observed in bronchiectasis patients compared to healthy controls.

Conclusions:

CD161 expression is a key marker for defining γδ T cell heterogeneity and their differentiation states.
The Vδ1+ terminally differentiated effector memory γδ T cell subset may be implicated in chronic inflammatory conditions such as bronchiectasis.