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Genetic Engineering Systems to Study Human Viral Pathogens from the Coronaviridae Family

S O Galkin1,2, A N Anisenko1,2,3, O A Shadrina2,3

  • 1Bioengineering and Bioinformatics Department, Moscow State University, 119991 Moscow, Russia.

Molecular Biology
|February 23, 2022
PubMed

Insights

This review summarizes genetic engineering systems for studying SARS-CoV-2 and other coronaviruses. It covers essential knowledge on coronavirus structure and life cycles for developing antiviral therapies.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetic Engineering

Background:

  • The COVID-19 pandemic, caused by SARS-CoV-2, highlighted the need for advanced cellular systems.
  • Studying coronaviruses requires effective methods for genome manipulation and inhibitor screening.

Purpose of the Study:

  • To review existing genetic engineering systems for studying SARS-CoV-2 and other Coronaviridae.
  • To provide foundational knowledge on coronavirus structure and life cycles.

Main Methods:

  • Literature review of genetic engineering systems for coronaviruses.
  • Compilation of data on SARS-CoV-2 and related viruses.
  • Summary of fundamental coronavirus biology.

Main Results:

  • Overview of various genetic engineering tools applicable to coronaviruses.
  • Discussion of systems enabling viral genome manipulation.
  • Identification of high-throughput screening platforms for antiviral discovery.

Conclusions:

  • Effective genetic engineering systems are crucial for combating coronavirus infections.
  • Understanding coronavirus structure and life cycles aids in developing novel therapeutic strategies.
  • This review consolidates key information for researchers in the field.
Keywords:
COVID-19SARS-CoV-2pseudovirusesreplicons

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