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Updated: Oct 2, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Y-shaped RNA secondary structure of a noncoding region in the genomic RNA of tick-borne encephalitis virus affects
Shoko Nishiyama1, Minato Hirano2,3, Memi Muto2
1Laboratory of Zoonotic Disease, Joint Department of Veterinary Medicine, Faculty of Applied Biosciences, Gifu University, Gifu, Japan.
Abstract:
Tick-borne encephalitis virus (TBEV) is a zoonotic virus that causes encephalitis in humans. Various deletions have been reported in a variable region of the 3' untranslated region of the TBEV genome. This study analyzed the role of a Y-shaped secondary structure in the pathogenicity of TBEV by using reverse genetics. Deletion of the structure increased the mortality rate of virus-infected mice but did not affect the virus multiplication in cultured cells and organs. The results indicate that the secondary structure is involved in the regulation of TBEV pathogenesis.
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