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Updated: Oct 2, 2025

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Oxygen-Varying Correlated Fluorescence for Determining the Stern-Volmer Constant of Porphyrin
Meng Kou1, Feng Qin2, Weiming Lv2
1School of Physics, Harbin Institute of Technology, Harbin, Heilongjiang 150001, People's Republic of China.
Abstract:
The achievement of the Stern-Volmer constant (KSV) of porphyrin, a parameter to describe the ability of energy transfer to oxygen, enables us to understand and engineer the functionalities of porphyrin. In this study, we propose a new method to obtain KSV by steady fluorescence based on the population correlation between the excited triplet state (T1) and the singlet excited state (S1). Nonlinear fluorescence intensity of porphyrin [hematoporphyrin monomethyl ether (HMME)] as a function of pump power is observed, and this nonlinearity is oxygen-concentration-dependent. These observations originated from the population correlation, where more intense pump power introduces a stronger population correlation. Most importantly, we have found that the oxygen content can influence this correlation via the quenching of T1 based on its sequent change of fluorescence intensity; KSV ∼ 28 kPa-1 can be obtained theoretically for HMME. Our investigation not only offers a promising method of achieving KSV conveniently by steady fluorescence but also enlightens the advances of regulable fluorescence correlation.
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