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Updated: Oct 2, 2025

Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
Published on: June 17, 2025
Sutcliffiella deserti sp. nov., isolated from desert soil
Pengze Bai1, Siyue Zhang1, Ya Chen1
1Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Abstract:
A Gram-stain-positive, facultative anaerobic, motile and rod-shaped bacterial strain, DG-18T, was isolated from desert soil sampled at the Kubuqi Desert in Inner Mongolia, China. Strain DG-18T grew at 4-40 °C (optimum, 25-30 °C), at pH 8.0-10.0 (optimum, pH 9.0) and with 0-8.0 % (w/v) NaCl (optimum 2.0%). 16S rRNA gene sequence analysis placed strain DG-18T within the genus Sutcliffiella of the family Bacillaceae with Sutcliffiella halmapala DSM 8723T (98.2%), Sutcliffiella zhanjiangensis JSM 099021T (97.6%), Sutcliffiella horikoshii DSM 8719T (97.4%), Sutcliffiella catenulata 18CT (96.6 %) and Sutcliffiella cohnii NBRC 15565T (96.5%) as its closest relatives. The major respiratory quinone of strain DG-18T was MK-7 and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Its major fatty acids were iso-C15 : 0 and iso-C17 : 1 ω10c. The genomic DNA G+C content of strain DG-18T was 38.7 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain DG-18T and that of S. halmapala DSM 8723T was 76.7 %. The Genome-to-Genome Distance Calculator showed that the DNA-DNA hybridization value for strain DG-18T and S. halmapala DSM 8723T was 21.8%. Based on the polyphasic taxonomic data, strain DG-18T represents a novel species of the genus Sutcliffiella, for which the name Sutcliffiella deserti sp. nov. is proposed. The type strain is DG-18T (=GDMCC 1.17773T=KCTC 43170T).
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