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Circ_0020123 promotes NSCLC tumorigenesis via up-regulating KIAA1522 expression through miR-940

Yihui Fan1, Qing Wang1, Minxin Shi1

  • 1Department of Thoracic Surgery, Tumor Hospital Affiliated to Nantong University, Nantong, Jiangsu Province, China.

Cell Cycle (Georgetown, Tex.)
|February 23, 2022
PubMed

Insights

Circular RNA circ_0020123 promotes non-small cell lung cancer (NSCLC) growth and spread. Targeting this oncogene via the miR-940/KIAA1522 pathway offers a potential therapeutic strategy for NSCLC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms driving NSCLC progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the role of circ_0020123 in NSCLC tumorigenesis.
  • To elucidate the molecular mechanism underlying circ_0020123's function in NSCLC.

Main Methods:

  • Quantitative real-time PCR to assess circ_0020123 expression.
  • Cell proliferation, migration, and invasion assays.
  • Western blotting and luciferase reporter assays to confirm molecular interactions.

Main Results:

  • Circ_0020123 was significantly upregulated in NSCLC tissues and cell lines.
  • Knockdown of circ_0020123 inhibited NSCLC cell proliferation, migration, and invasion in vitro and tumor growth in vivo.
  • Circ_0020123 directly targeted miR-940, and miR-940 targeted KIAA1522, forming the circ_0020123/miR-940/KIAA1522 axis.

Conclusions:

  • Circ_0020123 functions as an oncogene in NSCLC by regulating the miR-940/KIAA1522 pathway.
  • Circ_0020123 represents a potential diagnostic biomarker and therapeutic target for NSCLC.
Keywords:
KIAA1522NSCLCcirc_0020123miR-940tumorigenesis

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