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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
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Related Experiment Video

Updated: Oct 2, 2025

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
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SERS Tags for Biomedical Detection and Bioimaging.

Huiqiao Liu1, Xia Gao2, Chen Xu3

  • 1College of Chemistry and Chemical Engineering, Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis, Xinyang Normal University, Xinyang 464000, China.

Theranostics
|February 24, 2022
PubMed
Summary

Surface-enhanced Raman scattering (SERS) tags offer sensitive molecular identification for biosensing. These advanced SERS tags show great promise in biomedical applications, including disease diagnosis and in vivo imaging.

Keywords:
SERS tagbioimagingbiomarkerstheranosticstumor

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Area of Science:

  • Nanotechnology and Spectroscopy
  • Biomedical Engineering
  • Molecular Diagnostics

Background:

  • Surface-enhanced Raman scattering (SERS) is a powerful technique for sensitive molecular identification.
  • SERS offers high sensitivity, signal specificity, and photobleaching resistance, enabling diverse applications.
  • SERS tags, created by functionalizing plasmonic nanoparticles, provide multiplexing capabilities for biosensing.

Purpose of the Study:

  • To review recent advancements in SERS tags specifically for biomedical applications.
  • To highlight the development and utility of SERS tags in diagnostics, imaging, and theranostics.
  • To discuss the potential and challenges of SERS tags for future clinical translation.

Main Methods:

  • Review of literature focusing on the building blocks and recent progress of SERS tags.
  • Summarization of SERS tag applications in detecting biomarkers (DNA, miRNA, proteins) in biological fluids.
  • Analysis of SERS tag utility in various imaging modalities, from in vitro to in vivo studies.

Main Results:

  • SERS tags have demonstrated significant progress in detecting various biomarkers in biological samples.
  • Applications span from in vitro cell and bacteria imaging to in vivo tumor imaging and delineation.
  • SERS tags show potential for cancer diagnosis and delineating tumor margins.

Conclusions:

  • SERS tags are highly valuable tools in biomedical fields, offering advanced capabilities for detection and imaging.
  • The review underscores the growing importance of SERS tags in liquid biopsy, bioimaging, and theranostics.
  • Future perspectives and potential obstacles for clinical translation of SERS tags are discussed.