Ligature-induced periodontitis mouse model protocol for studying Saccharibacteria.

Area of Science:

• Oral microbiology
• Periodontal disease research
• Bacteriology

Background:

• Saccharibacteria (TM7) are unculturable bacteria with unknown pathogenicity.
• TM7 bacteria often exist as obligate symbionts on other bacteria.
• Studying TM7 pathogenicity requires specialized models due to their unique growth requirements.

Purpose of the Study:

• To establish a reproducible mouse model for investigating Saccharibacteria (TM7) pathogenicity.
• To evaluate the impact of TM7 on bone resorption and inflammation in a murine oral infection model.
• To provide a method for studying bacteria that do not readily colonize the mouse oral cavity.

Main Methods:

• Preparation of Saccharibacteria (TM7) for inoculation.
• Application of ligature with and without TM7 onto mouse molars.
• Measurement of bone resorption and inflammatory markers post-infection.

Main Results:

• The protocol allows for the controlled introduction of TM7 bacteria into the murine oral cavity.
• Quantifiable measurements of bone loss and inflammation associated with TM7 presence were obtained.
• The model demonstrated the feasibility of studying the pathogenic potential of TM7.

Conclusions:

• This ligature-based mouse model is effective for studying Saccharibacteria (TM7) pathogenicity.
• The model facilitates research into the role of TM7 in periodontal-like conditions.
• This protocol offers a valuable tool for investigating the oral microbiome and associated diseases.