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Updated: Oct 2, 2025
08:47
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Published on: March 15, 2021
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A protocol for full-rotation soft X-ray tomography of single cells
Jian-Hua Chen1,2, Bieke Vanslembrouck1,2, Valentina Loconte1,2
1Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
STAR Protocols
|February 24, 2022
Summary
This protocol details sample preparation, data acquisition, and cellular organelle segmentation using soft X-ray tomography. It is applicable to specific microscopes and sample holders, aiding in advanced cellular imaging.
Area of Science:
- Cell Biology
- Microscopy Techniques
- Biophysics
Background:
- Soft X-ray tomography (SXT) offers high-resolution imaging of cellular structures.
- Detailed protocols are crucial for reproducible SXT experiments.
- Understanding cellular organelle morphology requires advanced imaging methods.
Purpose of the Study:
- To provide a comprehensive protocol for soft X-ray tomography.
- To enable detailed segmentation of cellular organelles.
- To facilitate reproducible SXT data acquisition and analysis.
Main Methods:
- Step-by-step sample preparation for SXT.
- Full-rotation data acquisition strategies.
- Image segmentation techniques for identifying cellular organelles.
Main Results:
- A standardized protocol for SXT sample preparation and imaging.
- Methodology for accurate segmentation of cellular organelles.
- Demonstration of applicability to specific SXT systems.
Conclusions:
- The described protocol enables detailed visualization and analysis of cellular organelles.
- This method is adaptable for various SXT setups with similar sample holders.
- It serves as a valuable resource for researchers in cell biology and microscopy.
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