A protocol for full-rotation soft X-ray tomography of single cells
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Updated: Oct 2, 2025

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Published on: March 15, 2021
Jian-Hua Chen1,2, Bieke Vanslembrouck1,2, Valentina Loconte1,2
1Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Abstract:
The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).
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