Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial enzyme-responsive hydrogels for triggered delivery of antibiotics to infected wounds.

Science advances·2026
Same author

Dual Biomolecule Patterning on Micropatterned Polylactide Surfaces Bearing Oppositely Charged Polymer Brushes at Neighboring Sites.

ACS biomaterials science & engineering·2026
Same author

Dual-receptor targeting of type I dendritic cells with DNA-scaffolded nanoparticles enhances STING-licensed antitumor immunity.

Science advances·2026
Same author

Methylene Blue-Loaded Liposomal Nanocarriers Enhance the Efficacy of Photodynamic Therapy against <i>Candida auris</i> Biofilm.

ACS infectious diseases·2025
Same author

Microencapsulation of Liver Spheroids with Poly(Vinyl Alcohol) Enhances Function Compared with Alginate.

Tissue engineering. Part A·2025
Same author

Fungal Enzyme-Responsive Hydrogel Drug Delivery Platform for Triggered Antifungal Release.

Advanced healthcare materials·2024

Related Experiment Video

Updated: Oct 2, 2025

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

9.4K

Mesenchymal Stem Cell Behavior on Soft Hydrogels with Aligned Surface Topographies.

Akram Abbasi1, Sachiko Imaichi2, Vincent Ling2

  • 1School of Engineering, Center for Biomedical Engineering, Brown University, Providence, Rhode Island 02912, United States.

ACS Applied Bio Materials
|February 24, 2022
PubMed
Summary

Soft hydrogels with microgrooves improve human mesenchymal stem cell expansion for cell therapies. These substrates maintain cell multipotency and reduce senescence, enhancing therapeutic potential.

Keywords:
cytokinesdifferentiationmesenchymal stem cellsmultipotencypolyacrylamide hydrogelssurface topography

More Related Videos

Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.0K
Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel
13:28

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel

Published on: August 8, 2017

8.1K

Related Experiment Videos

Last Updated: Oct 2, 2025

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

9.4K
Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.0K
Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel
13:28

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel

Published on: August 8, 2017

8.1K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Regenerative Medicine

Background:

  • Human mesenchymal stem cells (HMSCs) are crucial for cell-based therapies.
  • Current HMSC expansion methods on stiff substrates cause senescence and reduce therapeutic efficacy.
  • Optimizing in vitro expansion is vital for successful HMSC therapies.

Purpose of the Study:

  • To develop novel 2D soft hydrogel substrates with microscale aligned grooves for HMSC culture.
  • To investigate the effect of these topographical features on HMSC behavior and therapeutic potential.
  • To enhance the multipotency, reduce senescence, and improve the secretome of expanded HMSCs.

Main Methods:

  • Fabrication of 2D soft hydrogels with imprinted microscale aligned grooves.
  • Culture of human mesenchymal stem cells on these novel hydrogel substrates.
  • Analysis of cellular morphology, cytoskeletal tension, multipotency, senescence, and secretome.

Main Results:

  • Soft hydrogels with specific topographical features reduced cellular spreading and cytoskeletal tension.
  • HMSCs cultured on these hydrogels maintained multipotency and differentiation potential (osteogenic and adipogenic).
  • Reduced cellular senescence and enhanced production of anti-inflammatory cytokines were observed in HMSCs.

Conclusions:

  • Soft hydrogels with microgroove topography are promising substrates for in vitro HMSC expansion.
  • These substrates preserve key stem cell characteristics and enhance therapeutic potential.
  • This approach offers a strategy to improve the efficacy of HMSC-based therapies.