Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Corrigendum to "Influence of polymer molecular weight on the in vitro cytotoxicity of poly (N-isopropylacrylamide)" [Mater. Sci. Eng. C (2016) 509-513].

Materials science & engineering. C, Materials for biological applications·2021

Same author

Highly Perfluorinated Covalent Triazine Frameworks Derived from a Low-Temperature Ionothermal Approach Towards Enhanced CO<sub>2</sub> Electroreduction.

Angewandte Chemie (International ed. in English)·2021

Same author

Atomically Dispersed High-Density Al-N<sub>4</sub> Sites in Porous Carbon for Efficient Photodriven CO<sub>2</sub> Cycloaddition.

Advanced materials (Deerfield Beach, Fla.)·2021

Same author

A Holistic Approach for Elucidating Local Structure, Dynamics, and Speciation in Molten Salts with High Structural Disorder.

Journal of the American Chemical Society·2021

Same author

Correction to "NIPAM-Based Microgel Microenvironment Regulates Cardiac Stem Cells".

ACS applied materials & interfaces·2021

Same author

Synthesis of Poly(ionic Liquid)s-<i>block</i>-poly(methyl Methacrylate) Copolymer-Grafted Silica Particle Brushes with Enhanced CO<sub>2</sub> Permeability and Mechanical Performance.

Langmuir : the ACS journal of surfaces and colloids·2021

Same journal

A Coumarin-Based Probe for Sequential ON-OFF-ON Detection of Cu<sup>2+</sup> and Biothiols: Naked-Eye Detection, Smartphone RGB Readout and In Vivo Imaging.

Biosensors·2026

Same journal

Electropolymerized Molecularly Imprinted Polymers Supported on Carbon-Based Materials for (Bio)sensing: Direct and Indirect Detection Strategies.

Biosensors·2026

Same journal

Progress in (Photo)electrochemical Biosensors for the Detection of Amyloid-Beta Oligomer.

Biosensors·2026

Same journal

Design and Simulation of Lamotrigine Intermittent Release from a Subcutaneous Implant with an Enzymatic Biosensor Based on Clinical Data.

Biosensors·2026

Same journal

Prediction of Chronic Kidney Disease Based on Simulated Serum Analysis by Vibrational Spectroscopy.

Biosensors·2026

Same journal

AI/ML-Assisted SERS Biosensing for Biomolecular Detection: From Direct Spectral Response to Integrated Diagnostic Systems.

Biosensors·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 2, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters.

Umar Azhar¹, Qazi Ahmed¹, Saira Ishaq¹

¹School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, Australia.

|February 24, 2022

Summary

This summary is machine-generated.

This study introduces a novel multiplex immunoassay using Raman-coded microbeads and SERS-coded nanotags for accurate, label-free detection of multiple analytes. This bead-based system offers a promising platform for advanced biomedical analysis.

Keywords:

Raman SERS immunoassays microbeads multiplex

More Related Videos

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Related Experiment Videos

Last Updated: Oct 2, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Area of Science:

Biotechnology
Analytical Chemistry
Nanotechnology

Background:

Multiplex bioassays require accurate detection of multiple analytes simultaneously.
Existing multiplex immunoassay systems often rely on complex encoding mechanisms.
Suspension microsphere immunoassays are emerging as powerful tools for multiplex analysis.

Purpose of the Study:

To develop a novel, label-free, bead-based multiplex immunoassay system.
To utilize Raman-coded antibody supports and SERS-coded nanotags for multiplex detection.
To demonstrate selective identification of paratope-epitope interactions in a single sample.

Main Methods:

Developed a homogeneous immunoassay using surface-functionalized, monodisperse Raman-coded microbeads (polystyrene and poly(4-tert-butylstyrene)).
Employed SERS-coded nanotags (organic thiols on gold nanoparticles) as reporters.
Utilized two distinct nanotags: 4-mercaptobenzoic acid and 3-mercaptopropionic acid.

Main Results:

Successfully created a multiplex Raman/SERS-based microsphere immunoassay system.
Demonstrated selective identification of specific paratope-epitope interactions from a mixture.
Achieved detection under a single laser illumination, highlighting efficiency.

Conclusions:

The developed assay is a fluorescent, label-free, bead-based multiplex immunoassay.
This novel system holds significant promise for future suspension multiplex analysis in diverse biomedical applications.
Offers a robust platform for simultaneous detection and analysis of multiple analytes.