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    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Diffractive Optics

    Background:

    • Microscope objectives traditionally face a trade-off between numerical aperture (NA) and field of view.
    • Diffractive lens arrays (DLAs) offer a potential solution by using overlapping apertures.

    Purpose of the Study:

    • To develop and demonstrate a diffractive lens array (DLA) system for enhanced microscopy.
    • To overcome the conventional NA-field of view trade-off in optical microscopy.

    Main Methods:

    • Fabrication and characterization of a DLA producing a spot array.
    • Implementation of a DLA-based confocal microscopy setup.
    • Imaging of fluorescence beads and demonstration of aberration insensitivity.

    Main Results:

    • The DLA generated a spot array with a numerical aperture (NA) up to 0.83 and a pitch of 75 µm at 488 nm wavelength.
    • The DLA-based confocal setup achieved high-resolution measurements over a 3mm x 3mm area using a 2.5x0.07 NA objective.
    • The fluorescence microscope demonstrated robustness against optical aberrations.

    Conclusions:

    • DLAs can effectively overcome the NA-field of view limitation in microscopy.
    • The proposed DLA-based fluorescence microscope offers high-resolution, large-area imaging capabilities.
    • The system's insensitivity to optical aberrations broadens its practical applications in microscopy.