Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

8.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enabling Fluoroalkyl-Sulfonylalkylation and Fluoroalkyl-Halogenation of Alkenes and Alkynes via Photoredox Catalysis.

Journal of the American Chemical Society·2026
Same author

Generation of Mono- and Bisazabicyclic <i>o</i>-Quinodimethanes through Polarity-Matched and -Mismatched 6π-Azaelectrocyclization: Access to Substituted Naphthalenes.

Organic letters·2026
Same author

An I<sub>2</sub>-Catalyzed, Visible-Light Photoredox-Mediated Radical Conversion of α-Nitrocarbonyls to Nitrile Oxides: Catalytic Access to Isoxazolines and Isoxazoles from Alkenes and Alkynes.

Organic letters·2025
Same author

Ni-Catalyzed Regioselective Alkylarylation of Unactivated Alkenes in Amines Enabled by Cooperative Ligand Effects of Nitriles and Electron-Deficient Alkenes.

Journal of the American Chemical Society·2025
Same author

Catalytic Photoredox Carbobromination of Unactivated Alkenes with α-Bromocarbonyls via the Mechanistically Distinct Radical-Addition Radical-Pairing Pathway.

ACS catalysis·2025
Same author

Robotic-assisted carinal reconstruction using cross-table ventilation.

Multimedia manual of cardiothoracic surgery : MMCTS·2024

Related Experiment Video

Updated: Oct 2, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.4K

Backscatter multiple wavelength digital holography for color micro-particle imaging.

Ramesh Giri, Matthew J Berg

    Applied Optics
    |February 24, 2022
    PubMed
    Summary

    Digital holography now images micro-particles in color using a Michelson interferometer and three wavelengths. This technique reconstructs color images objectively, similar to conventional microscopy.

    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Particle Imaging

    Background:

    • Digital holography typically produces monochrome images.
    • Color imaging of micro-particles is crucial for material science and diagnostics.
    • Existing color microscopy methods may have limitations in resolution or sample manipulation.

    Purpose of the Study:

    • To develop a digital holography technique for full-color imaging of stationary micro-particles.
    • To achieve color reconstruction without spectral crosstalk.
    • To enable quantitative color analysis of micro-particles.

    Main Methods:

    • Utilized a Michelson interferometer setup with three primary wavelengths (430, 532, 633 nm).
    • Employed a three-CMOS prism sensor for simultaneous, spectrally resolved hologram recording.

    More Related Videos

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K
    Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
    14:58

    Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

    Published on: June 2, 2010

    9.7K

    Related Experiment Videos

    Last Updated: Oct 2, 2025

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.4K
    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K
    Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
    14:58

    Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

    Published on: June 2, 2010

    9.7K
  • Applied Fresnel diffraction theory for image reconstruction and additive color mixing.

    • Main Results:

    • Successfully generated color images of micro-particles, comparable to white-light microscopy.
    • Demonstrated feasibility with colored micro-spheres and dust particles.
    • Implemented speckle-noise suppression and white balance for improved image quality.

    Conclusions:

    • The proposed digital holography method enables objective, quantitative color imaging of micro-particles.
    • The technique offers a viable alternative to conventional color microscopy for particle analysis.
    • Chromaticity analysis provides a robust method for differentiating particle colors.