Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structure-dependent thermochromism of PAZO thin films: theory and experiment.

Beilstein journal of nanotechnology·2026

Same author

The Synthesis of New Chalcogenides from the System GeTe<sub>6</sub>-Cu and a Layered Structure Based on Them and an Azo Polymer for Application in Optoelectronics.

Materials (Basel, Switzerland)·2025

Same author

Reconstruction of Optical Coherence Tomography Images from Wavelength Space Using Deep Learning.

Sensors (Basel, Switzerland)·2025

Same author

Density Functional Theory Prediction of Laser Dyes-Cucurbit[7]uril Binding Affinities.

Molecules (Basel, Switzerland)·2024

Same author

Transfer Learning-Based Approach for Thickness Estimation on Optical Coherence Tomography of Varicose Veins.

Micromachines·2024

Same author

Nanocomposite Photoanisotropic Materials for Applications in Polarization Holography and Photonics.

Nanomaterials (Basel, Switzerland)·2023

Search research articles

Related Experiment Video

Updated: Oct 2, 2025

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Published on: August 5, 2009

Intensity-based dynamic speckle method using JPEG and JPEG2000 compression.

Elena Stoykova, Blaga Blagoeva, Natalya Berberova-Buhova

|February 24, 2022

Summary

This summary is machine-generated.

Lossy compression using JPEG and JPEG2000 formats can be effectively applied to dynamic speckle analysis. This method allows for efficient storage and processing of large image datasets without compromising speed distribution analysis.

More Related Videos

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences

Published on: June 16, 2014

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

Published on: December 3, 2018

Related Experiment Videos

Last Updated: Oct 2, 2025

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Published on: August 5, 2009

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences

Published on: June 16, 2014

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

Published on: December 3, 2018

Area of Science:

Optics and Photonics
Data Processing and Analysis

Background:

Dynamic speckle analysis uses statistical processing of speckle patterns to observe process speeds.
Monitoring temporal changes requires handling large image datasets, posing storage and processing challenges.

Purpose of the Study:

To investigate the feasibility of using lossy compression techniques (JPEG, JPEG2000) for dynamic speckle analysis.
To assess the impact of compression on the accuracy of speed distribution maps derived from speckle data.

Main Methods:

Implementation of JPEG and JPEG2000 lossy compression algorithms on image sequences.
Generation of speed distribution maps from both original and compressed/decompressed speckle images.
Comparison of analysis results from compressed and uncompressed data using synthetic and experimental images.

Main Results:

Lossy compression using JPEG and JPEG2000 formats was successfully applied to dynamic speckle analysis.
Decompressed images yielded comparable speed distribution maps to non-compressed images.
Both compression formats proved suitable for processing large image datasets in dynamic speckle analysis.

Conclusions:

Lossy compression is a viable method for managing large datasets in dynamic speckle analysis.
JPEG and JPEG2000 compression do not significantly impair the accuracy of speed mapping.
This approach facilitates efficient monitoring of time-evolving processes using dynamic speckle techniques.