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Involvement of transcribed lncRNA uc.291 and SWI/SNF complex in cutaneous squamous cell carcinoma
M Mancini1, A Cappello2, R Pecorari2
1Istituto Dermopatico Dell'Immacolata-IRCCS, via dei Monti di Creta 104, 00167, Rome, Italy.
Discover Oncology
|February 24, 2022
Summary
Down-regulation of uc.291 and BRG1 in cutaneous squamous cell carcinoma (cSCC) alters chromatin accessibility, leading to de-differentiation. This finding may identify new biomarkers for aggressive skin cancers.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Non-melanoma skin cancers (NMSCs) are common, but cutaneous squamous cell carcinoma (cSCC) can be lethal.
- A pathway involving lncRNA uc.291 and ACTL6A regulates epidermal differentiation genes.
- Transcribed ultra-conserved regions (T-UCRs) are implicated in tumorigenesis.
Purpose of the Study:
- To investigate the role of uc.291 dysregulation in cSCC de-differentiation.
- To analyze expression patterns of uc.291 and related proteins in human cSCC tumors.
- To explore potential clinical biomarkers for cSCC.
Main Methods:
- Analysis of mRNA and protein expression in human cSCC tumor biopsies.
- Examination of chromatin accessibility of epidermal differentiation complex genes.
- Review of publicly available datasets for SCCs of various origins.
Main Results:
- Down-regulation of uc.291 and BRG1 expression was observed in cSCC.
- Altered chromatin accessibility of epidermal differentiation genes correlates with de-differentiation.
- Findings suggest a general role for this axis in SCCs across different origins.
Conclusions:
- Down-regulation of uc.291 and BRG1 contributes to keratinocyte malignancy and de-differentiation in cSCC.
- This pathway offers potential for identifying novel clinical biomarkers for cutaneous SCC.
- The observed mechanism may be relevant for SCCs from various anatomical sites.
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