Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.8K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.8K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Defense Mechanism Against Infection01:26

Defense Mechanism Against Infection

7.3K
Natural flora, body system defenses, and inflammation are natural barriers of the body against infectious agents regardless of previous exposure. Normal floras of the human body refer to the microbial population that colonizes the skin and mucous membranes.
In addition, many body organ systems have unique defenses against infection. The skin is an intact, multilayered surface preventing invasion by microorganisms unless impaired. Mucous membranes lining the mouth, nose, and eyelids are barriers...
7.3K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

74.7K
Overview
74.7K
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy01:30

Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy

443
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
443

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Type 3 fimbrial regulation underpins anti-MrkA immunotherapeutic efficacy in experimental Klebsiella pneumoniae infection.

The Journal of infectious diseases·2026
Same author

Phospholipid-independent biogenesis and function of the RP4 conjugation pilus.

Nature communications·2026
Same author

Lymph node microenvironment remodeling unlocks local antibody-mediated B cell depletion.

Science advances·2026
Same author

GBP1 recruitment to actin-rich pedestals of extracellular Gram-negative bacteria promotes pyroptosis.

The EMBO journal·2026
Same author

Variability in the TLR3 type I interferon pathway is predictive of RNA vaccine responses.

Science advances·2026
Same author

Plug-and-play evolution of the Klebsiella pneumoniae capsule locus enables serotype exchange across genetic backgrounds.

PLoS biology·2026

Related Experiment Video

Updated: Oct 2, 2025

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
07:34

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

2.8K

Trained ILC3 responses promote intestinal defense.

Nicolas Serafini1, Angélique Jarade1, Laura Surace1

  • 1Institut Pasteur, Université de Paris, Inserm U1223, Innate Immunity Unit, Paris, France.

Science (New York, N.Y.)
|February 24, 2022
PubMed
Summary

Group 3 innate lymphoid cells (ILC3s) develop lasting memory after infection. These trained ILC3s offer enhanced immune protection against future pathogen challenges.

More Related Videos

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
08:22

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

5.9K
Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
08:00

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Published on: June 24, 2019

8.2K

Related Experiment Videos

Last Updated: Oct 2, 2025

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
07:34

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

2.8K
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
08:22

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

5.9K
Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
08:00

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Published on: June 24, 2019

8.2K

Area of Science:

  • Immunology
  • Innate Immunity
  • Cellular Immunology

Background:

  • Group 3 innate lymphoid cells (ILC3s) are crucial innate immune cells involved in host defense, particularly at mucosal surfaces.
  • The long-term functional plasticity of ILC3s following pathogen exposure remains largely uncharacterized.

Purpose of the Study:

  • To investigate whether intestinal ILC3s undergo stable functional modifications after a defined encounter with an enterobacterial pathogen.
  • To determine if these modified ILC3s exhibit enhanced responses upon subsequent pathogen challenge.

Main Methods:

  • Mice were challenged with the enterobacterium *Citrobacter rodentium* to induce a time-restricted infection.
  • Intestinal ILC3 populations were analyzed for phenotypic and functional changes over extended periods post-infection.
  • Rechallenge experiments were performed to assess the responses of previously exposed ILC3s compared to naive ILC3s.

Main Results:

  • Intestinal ILC3s remained in an activated state for months after *C. rodentium* infection.
  • Upon rechallenge, these "trained" ILC3s demonstrated increased proliferation and enhanced production of interleukin-22 (IL-22).
  • Trained ILC3s exhibited a superior ability to control infection compared to naive ILC3s, linked to metabolic and proliferative enhancements.

Conclusions:

  • A limited exposure to *Citrobacter rodentium* induces durable phenotypic and functional adaptations in intestinal ILC3s.
  • These adaptive changes in ILC3s contribute to enhanced long-term mucosal immunity and host defense.
  • This study reveals a form of immunological memory within the innate immune system mediated by ILC3s.