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Related Concept Videos

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Related Experiment Video

Updated: Oct 2, 2025

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
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Multiplexed Genome Editing for Efficient Phenotypic Screening in Zebrafish.

Shuyu Guo1, Ge Gao1, Cuizhen Zhang1

  • 1State Key Laboratory of Medical Neurobiology, Ministry of Education Frontiers Center for Brain Science, and Institution of Brain Science, Fudan University, Shanghai 200032, China.

Veterinary Sciences
|February 24, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a faster way to create zebrafish gene mutants using multiplexed genome editing. This method identified a new candidate gene, tmem183a, linked to hearing loss in zebrafish.

Keywords:
CRISPR-Cas9hearing lossmultiplexed genome editingtmem183azebrafish

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Area of Science:

  • Genetics and Genomics
  • Developmental Biology
  • Neuroscience

Background:

  • Zebrafish are valuable models for studying human disease genes.
  • CRISPR-Cas9 gene editing is powerful but limited by single guide RNA efficiency.
  • Multiplexed genome editing offers enhanced efficiency and broader applications in genetic research.

Purpose of the Study:

  • To develop an efficient multiplexed genome editing strategy for generating zebrafish mutants.
  • To establish a robust genetic platform for rapid zebrafish mutant generation.
  • To identify novel candidate genes for human diseases using zebrafish models.

Main Methods:

  • Employing a multiplexed genome editing strategy in zebrafish.
  • Utilizing CRISPR-Cas9 technology for efficient gene editing.
  • Conducting behavioral tests and histological examinations for phenotypic analysis.

Main Results:

  • Successfully generated zebrafish mutants using the described multiplexed genome editing strategy.
  • Identified a novel candidate gene, tmem183a, associated with hearing loss.
  • Demonstrated the efficiency and scope of multiplexed editing for genetic studies.

Conclusions:

  • The developed multiplexed genome editing strategy provides a robust and efficient platform for zebrafish mutant generation.
  • This approach accelerates the identification of candidate genes for human diseases.
  • The study highlights the potential of tmem183a as a candidate gene for hearing loss.