Related Experiment Video
Updated: Oct 2, 2025
05:34
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
Published on: September 8, 2023
925
Isothermal amplification-assisted diagnostics for COVID-19.
Mariarita De Felice1, Mariarosaria De Falco1, Daniele Zappi2
1Institute of Bioscience and BioResources, National Research Council, Department of Biology, Agriculture and Food Science, Via P. Castellino 111, 80100, Naples, Italy.
Biosensors & Bioelectronics
|February 24, 2022
Summary
Rapid advancements in molecular diagnostics for infectious diseases, including COVID-19, focus on sensitive, specific, and rapid isothermal nucleic acid amplification. These portable biosensors offer efficient point-of-need screening, especially in low-resource settings.
Area of Science:
- Molecular diagnostics
- Infectious disease detection
- Biosensing technologies
Background:
- The COVID-19 pandemic highlighted the need for rapid and accurate infectious disease diagnostics.
- Existing diagnostic methods face challenges in sensitivity, specificity, speed, and accessibility.
Purpose of the Study:
- To review the latest trends in isothermal nucleic acid amplification-powered biosensors for detecting SARS-CoV-2.
- To highlight the advantages and disadvantages of these emerging diagnostic tools.
Main Methods:
- Integration of isothermal nucleic acid amplification with advanced biosensing systems.
- Development of portable and user-friendly diagnostic devices.
- Focus on achieving high sensitivity and specificity at the point of need.
Main Results:
- Isothermal amplification enables rapid and efficient nucleic acid sequence accumulation at a constant temperature.
- Portable biosensors enhance sensitivity and enable screening in low-resource settings.
- Various configurations of isothermal amplification-powered biosensors for SARS-CoV-2 detection have been developed.
Conclusions:
- Emerging diagnostic devices utilizing isothermal amplification and biosensing offer a promising solution for infectious disease surveillance.
- These technologies address critical needs for speed, accuracy, and accessibility in molecular diagnostics.
- Further development is crucial for widespread adoption in diverse healthcare settings.
