Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Local Anesthetics: Mechanism of Action01:23

Local Anesthetics: Mechanism of Action

Local anesthetics (LAs) block sensory and motor impulses by inhibiting the sodium channels on the nerve cell membranes. This induces temporary loss of sensation, relieving pain in a specific body area.
Local anesthetics are amphiphilic molecules consisting of a hydrophobic aromatic part linked to a hydrophilic group by an ester or amide linkage. They are weak bases and are usually available as salts, which increases their solubility and stability. Once administered, LAs exist in the body either...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monitoring T-cell function in septic shock: one-year experience with a fully automated assay.

Critical care (London, England)·2026
Same author

Pelvic embolization in severely traumatized patients in hemorrhagic shock with pelvic fractures without contrast extravasation on the initial CT scan: A retrospective comparative study between three French centers.

Injury·2026
Same author

Effectiveness of high-sensitivity troponin I to predict cardiovascular events in chest trauma patients.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2026
Same author

MNX1 prevents somatostatin expression in human beta cells by repressing PERCC1.

Diabetologia·2025
Same author

Epidemiology of pain, delirium, psychiatric disorders, discomfort and sedation-analgesia management in the intensive care unit: a one-day nationwide study.

Critical care (London, England)·2025
Same author

Considering local immunity for innovative immunomodulatory approaches: pulmonary sepsis as a use case.

Frontiers in immunology·2025

Related Experiment Video

Updated: Jun 25, 2026

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain
07:52

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

Published on: July 7, 2015

8.3K

Using Inhibitory DREADDs to Silence LC Neurons in Monkeys.

Pauline Perez1, Estelle Chavret-Reculon2, Philippe Ravassard2,3

  • 1Motivation, Brain and Behavior Team, Institut du Cerveau (ICM), INSERM UMRS 1127, CNRS UMR 7225, Pitié-Salpêtrière Hospital, 75013 Paris, France.

Brain Sciences
|February 25, 2022
PubMed
Summary

Researchers developed a novel DREADD method to reversibly inactivate the locus coeruleus (LC) in monkeys. This technique allows precise control over LC neuron activity, crucial for studying its role in cognition and behavior.

Keywords:
chemogeneticslocus coeruleusneuronal inhibitionnoradrenalineprimates

More Related Videos

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning
09:22

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning

Published on: June 22, 2015

14.8K
Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity
08:28

Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity

Published on: August 25, 2019

13.8K

Related Experiment Videos

Last Updated: Jun 25, 2026

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain
07:52

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

Published on: July 7, 2015

8.3K
A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning
09:22

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning

Published on: June 22, 2015

14.8K
Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity
08:28

Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity

Published on: August 25, 2019

13.8K

Area of Science:

  • Neuroscience
  • Primate Research
  • Molecular Biology

Background:

  • The locus coeruleus (LC) plays a vital role in cognition and behavior, including attention, decision-making, and stress responses.
  • Viral tools have advanced rodent brain research, but primate-specific tools are essential for translational applications.
  • Understanding LC function in primates is critical for exploring clinical strategies for neurological disorders.

Purpose of the Study:

  • To develop a selective and reversible method for inactivating locus coeruleus (LC) neurons in monkeys.
  • To investigate the dose-dependent effects of LC inactivation on cognitive functions.
  • To establish a valuable tool for primate research on LC function.

Main Methods:

  • Utilized a pharmacogenetic approach employing Designer Receptors Exclusively Activated by Designer Drugs (DREADD).
  • Expressed the hM4Di DREADD specifically in noradrenergic LC neurons.
  • Administered varying doses of the DREADD activator, deschloroclozapine (DCZ), to modulate LC neuron activity.

Main Results:

  • Successfully restricted hM4Di DREADD expression to noradrenergic LC neurons.
  • Demonstrated that DCZ dose correlates with the degree of LC neuron inhibition.
  • High DCZ doses (>0.3 mg/kg) caused significant LC inhibition and decreased vigilance.
  • Lower DCZ doses (<0.3 mg/kg) moderately reduced LC activity without affecting vigilance, enabling cognitive assessments.

Conclusions:

  • The DREADD approach offers selective and reversible control over LC neuron activity in monkeys.
  • Dose-dependent modulation of LC activity allows for the study of its role in subtle cognitive processes like attention and decision-making.
  • This pharmacogenetic tool is essential for advancing primate research on LC function and its translational implications.