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Published on: August 10, 2018
miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
Xin Yang1,2,3, Zhenhui Li1,2,3, Zhijun Wang1,2,3
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Lingnan Guangdong Laboratory of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Abstract:
As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find that miR-27b-3p and Cbl-b genes are significantly differentially expressed in the induced atrophy model. The dual-luciferase experiment and Western blot analysis confirmed that miR-27b-3p could regulate the expression of Cbl-b. In C2C12-differentiated myotubes, the overexpression of the Cbl-b gene showed that Cbl-b could upregulate the expression of MuRF-1 and Atrogin-1, which are related marker genes of muscle atrophy, at both the mRNA and protein levels, indicating that the Cbl-b gene can specifically affect muscle atrophy. The knockdown of the Cbl-b gene after C2C12-differentiated myotubes induced atrophy treatment can downregulate the expression of muscle-atrophy-related genes, indicating that manual intervention to downregulate the expression of Cbl-b has a certain alleviating effect on muscle atrophy. These data suggest that miR-27b-3p can regulate the expression of the Cbl-b gene and then exert a particular influence on muscle atrophy through the Cbl-b gene.
Insights
MicroRNAs regulate muscle atrophy. This study shows miR-27b-3p targets Cbl-b, influencing muscle atrophy marker genes MuRF-1 and Atrogin-1, suggesting therapeutic potential.
Area of Science:
- Molecular Biology
- Muscle Physiology
Background:
- Muscle atrophy involves increased protein degradation and decreased synthesis.
- MicroRNAs are key regulators of muscle atrophy, attracting significant research interest.
Purpose of the Study:
- To investigate the role of miR-27b-3p in muscle atrophy.
- To elucidate the regulatory relationship between miR-27b-3p and Cbl-b in muscle atrophy.
Main Methods:
- Differential gene expression analysis in an induced atrophy model.
- Dual-luciferase reporter assays to confirm miR-27b-3p regulation of Cbl-b.
- Western blot and RT-qPCR to assess gene and protein expression in C2C12 myotubes.
Main Results:
- miR-27b-3p and Cbl-b were significantly differentially expressed in muscle atrophy.
- miR-27b-3p directly regulates Cbl-b expression.
- Cbl-b overexpression upregulated muscle atrophy markers (MuRF-1, Atrogin-1); Cbl-b knockdown alleviated atrophy markers.
Conclusions:
- miR-27b-3p influences muscle atrophy by regulating Cbl-b.
- Cbl-b plays a specific role in muscle atrophy by modulating MuRF-1 and Atrogin-1.
- Downregulating Cbl-b shows potential for alleviating muscle atrophy.
