Related Experiment Video
Updated: Oct 2, 2025
10:55
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
Published on: March 21, 2022
2.3K
Regulation of p53 Function by Formation of Non-Nuclear Heterologous Protein Complexes
Lev Zavileyskiy1, Victoria Bunik1,2,3
1Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia.
Biomolecules
|February 25, 2022
Summary
The tumor suppressor p53 protein forms complexes outside the nucleus, influencing cellular stress responses and functions. Understanding these p53 complexes offers new therapeutic targets for fine-tuning cellular programs.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- The transcription factor p53 is a crucial stress-response protein.
- p53's functions are modulated by interactions with various proteins.
- p53 is found in multiple cellular compartments beyond the nucleus.
Purpose of the Study:
- To investigate non-nuclear p53 heterologous protein complexes.
- To explore the structural determinants and post-translational modifications of these complexes.
- To elucidate the role of these complexes in p53's intricate functions.
Main Methods:
- Analysis of p53 protein-protein interactions in various cellular compartments.
- Characterization of structural and regulatory aspects of p53 complexes.
- Investigation of p53 complex involvement in cellular stress responses and redox regulation.
Main Results:
- Identified numerous non-nuclear p53 complexes with regulatory and metabolic proteins.
- Demonstrated that some complexes sequester p53 or its partners, while others form upon p53 upregulation.
- Highlighted the role of specific complexes in regulating p53's post-translational modifications and redox sensitivity.
Conclusions:
- Non-nuclear p53 complexes significantly contribute to cellular stress management and p53 regulation.
- Understanding these complexes provides insights into p53's diverse roles.
- Targeting p53 protein-protein interactions could lead to novel therapeutic strategies.
Related Concept Videos
02:04Covalently Linked Protein Regulators
7.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
7.6K
02:23Abnormal Proliferation
4.7K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
01:23Negative Regulator Molecules
36.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.4K
02:37DNA Damage can Stall the Cell Cycle
9.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.4K
01:19Interactions Between Signaling Pathways
6.6K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.6K
01:57Protein Complexes with Interchangeable Parts
2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K