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Quantitative Real-Time Analysis of Differentially Expressed Genes in Peripheral Blood Samples of Hypertension

Fawad Ali1,2, Arifullah Khan1, Syed Aun Muhammad3

  • 1Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad 44000, Pakistan.

Genes
|February 25, 2022
PubMed

Insights

This study identified seven key genes linked to hypertension, revealing how their altered expression contributes to cardiovascular issues and disease. Findings may guide new hypertension treatment strategies.

Area of Science:

  • Genomics and Molecular Biology
  • Cardiovascular Research
  • Systems Biology

Background:

  • Hypertension (HTN) is a major global risk factor for cardiovascular disease, stroke, and mortality.
  • Understanding the genetic underpinnings of HTN is crucial for developing effective treatments.

Purpose of the Study:

  • To identify differentially expressed genes (DEGs) involved in the pathophysiology of hypertension.
  • To analyze the biological functions and regulatory mechanisms of these DEGs.

Main Methods:

  • Utilized a system-level framework to analyze 22 public cDNA Affymetrix datasets for HTN.
  • Performed Gene Ontology (GO) and pathway enrichment analyses.
  • Validated gene expression profiles using quantitative polymerase chain reaction (qPCR).

Main Results:

  • Identified seven significant hypertension-related genes: ADM, ANGPTL4, USP8, EDN1, NFIL3, MSR1, and CEBPD.
  • Discovered enriched pathways related to HIF-1-α transcription, endothelin, and GPCR-binding ligands.
  • Observed significant upregulation of ADM, ANGPTL4, USP8, and EDN1, and downregulation of CEBPD, MSR1, and NFIL3.

Conclusions:

  • Aberrant expression of identified DEGs is associated with hypertension development and cardiovascular abnormalities.
  • These findings offer insights into potential therapeutic targets for modulating hypertension.

Keywords:
cDNA datasetsdifferentially expressed genesenrichment analysisexpression profilinghypertensionqPCR

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