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Updated: Oct 2, 2025

Author Spotlight: In Vitro Co-Culture System of Pine Shoots and Pinewood Nematode for Studying Host Volatile Response
Published on: September 27, 2024
Nematodes in the Pine Forests of Northern and Central Greece
Maria Karmezi1,2, Alkmini Bataka1, Dimitrios Papachristos3
1Forest Research Institute, Hellenic Agricultural Organization Demeter, Vassilika, 57006 Thessaloniki, Greece.
Abstract:
In the context of plants or plant products protection by harmful organisms, measures have been taken by EU countries in order to prevent their introduction and establishment into the EU, and also limit their expansion in case they do enter. Such a case is Bursaphelenchus xylophilus (Parasitaphelenchidae, Nematoda), already recorded in Portugal and Spain. So, Member States should take all the appropriate steps in order to monitor and confine if necessary susceptible plants and/or plant products. Such measures include annual surveys even in countries where pine wilt disease does not occur yet. Therefore, national survey programs are widely established, sampling and examining samples from pine trees showing suspicious symptoms that could potentially be attributed to B. xylophilus. In this direction, such a network has also been established in Greece collecting and examining wood samples nationwide. In total, 123 wood samples were collected from conifer trees of Northern and Central Greece. Though B. xylophilus was absent from all samples examined, four other Bursaphelenchus species were identified. In addition, other nematode taxa were also recorded, including several phytophagous, microbivorous as well as predatory nematode species. This highlights the fact that besides preventing the introduction of B. xylophilus in Greece, national survey programs can significantly contribute to and enhance our knowledge of the indigenous nematode species.
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