ZIF-8 Nanoparticles Based Electrochemical Sensor for Non-Enzymatic Creatinine Detection

Area of Science:

• Electrochemistry
• Materials Science
• Biomedical Engineering

Background:

• Creatinine is a key biomarker for kidney and muscle function.
• Existing creatinine sensors often lack sensitivity, stability, or cost-effectiveness.
• Development of novel, reliable creatinine detection methods is crucial.

Purpose of the Study:

• To develop a highly sensitive, selective, and non-enzymatic electrochemical sensor for creatinine detection.
• To utilize zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) for enhanced sensing capabilities.
• To validate the sensor's performance in clinical samples.

Main Methods:

• Modification of indium tin oxide (ITO) substrate with poly(3,4 ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) and ZIF-8 NPs.
• Characterization of the sensing electrode using FESEM, AFM, XRD, and XPS.
• Electrochemical analysis (amperometry and impedance) to evaluate sensing performance.
• Detection of creatinine via its interaction with ZIF-8 NPs, leveraging peroxidase-like activity.

Main Results:

• The sensor demonstrated high sensitivity and selectivity for creatinine.
• A low limit of detection (LOD) of 30 µM was achieved within a clinically relevant linear range (0.05 mM-2.5 mM).
• Excellent recovery rates were observed when testing the sensor with human serum samples.
• The ZIF-8 based sensor showed high selectivity against other biomolecules.

Conclusions:

• The developed ZIF-8 based non-enzymatic electrochemical sensor is highly sensitive and selective for creatinine.
• This sensor shows significant potential for accurate diagnosis of kidney and muscle-related disorders.
• The sensor's performance in human serum validates its clinical applicability and potential for detecting other renal markers.