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Published on: July 8, 2021
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Cytotoxic Potential of Denture Adhesives on Human Fibroblasts-In Vitro Study
Ewa Sobolewska1, Piotr Makowiecki2, Justyna Drozdowska3
1Department of Dental Prosthetics, Pomeranian Medical University, 70-111 Szczecin, Poland.
Materials (Basel, Switzerland)
|February 25, 2022
Summary
Denture adhesives can harm human fibroblast cells, significantly reducing cell viability in laboratory tests. Further research is needed to determine if these effects occur in patients using denture creams.
Area of Science:
- Biomaterials Science
- Cell Biology
- Dental Materials
Background:
- Denture adhesives are widely used for stabilizing removable dentures.
- Increased availability of these products necessitates understanding their biological effects.
Purpose of the Study:
- To evaluate the in vitro cytotoxicity of three common denture adhesives.
- To assess the impact of denture adhesives on human fibroblast viability and morphology.
Main Methods:
- Human fibroblast cultures were exposed to three denture adhesives.
- Cell viability was quantified using the trypan blue dye exclusion assay.
- Microscopic intracellular alterations were analyzed after staining with hematoxylin and eosin.
Main Results:
- All tested denture adhesives exhibited significant cytotoxic effects on human fibroblasts.
- Quantitative analysis showed a 45.87-61.13% reduction in cell viability (p=0.0001).
- Qualitative evaluation indicated slight to moderate cytotoxicity.
Conclusions:
- Denture adhesive creams demonstrate a clear toxic effect on human fibroblasts in vitro.
- Further in vivo studies are required to ascertain the clinical relevance of these findings in patients.
